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The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description.Animals are typically ready to ship in 11-14 weeks.This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.phi C31-recombination of Gt(ROSA)26Sor We will fulfill your order by providing at least two carriers for each strain ordered.Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. These Ai14 mice on a B6;129S6 mixed genetic background are designated Stock No. Because this Ai14 conditional allele has the att B/att P-flanked sequences deleted, it may also be called Ai14D, Ai14(RCL-td T)-D or Rosa-CAG-LSL-td Tomato-WPRE::delta Neo.The Rosa-CAG-LSL-td Tomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a lox P-flanked STOP cassette (with stop codons in all 3 reading frames and a triple poly A signal), td Tomato sequence (a non-oligomerizing Ds Red fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the m RNA transcript stability), a poly A signal, and an att B/att P-flanked PGK-FRT-Neo-poly A cassette.
To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ai14 mice homozygous for this Rosa-CAG-LSL-td Tomato-WPRE conditional allele (also called Ai14D, Ai14(RCL-td T)-D or Rosa-CAG-LSL-td Tomato-WPRE::ΔNeo) are viable and fertile.
A lox P-flanked STOP cassette is designed to prevent transcription of the red fluorescent protein variant td Tomato (see below).
We cannot guarantee the reproductive success of mice shipped to your facility.
If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract.
These Ai14 mice are useful as a Cre reporter strain - expressing td Tomato fluorescence following Cre-mediated recombination.